Stains
The most commonly utilised stain in microbiological laboratories is the
Gram stain, developed by Danish Microbiologist Christian Gram. The Gram
stain has become one of the most important tools in bacteriology taxonomy,
dividing bacteria into gram positive (staining blue) and gram negative
(staining red with counter stain).
Some years ago, reacting to inquiries from medical and food industry
bacteriological laboratories Fronine released a range of Gram stain reagents
that could be purchased as a complete set or individual reagents as required.
Fronine will custom make Gram stains to your tried and tested recipes.
This will mean that the staff whose job it is to microscopically examine
the gram stained preparations will see no change in the quality and appearance,
maximising their valuable time.
Fronine has a complete range of bacteriological stains, including AFB
(acid-fast bacilli) stains, stains for fungal elements, ova, cysts and
parasites.
- Alberts Stain A
- Alberts Stain B
- Crystal Violet
- Carbol Fuchsin Concentrated and Dilute
- Gentian Violet
- Grams Iodine
- Jensen’s Iodine
- Loeffler’s Methylene Blue
- Methyl Violet
- Neutral Red
- Safranin
- Urinary deposit stain
- Fluorescent stain for TB Auromine Rhodamine Stain
- Gomories Trichrome – Parasitology
- Trichrome Blue Stain – Parasitology
- Wheatons Trichrome – Parasitology
- Parasitology Stain Kit – Proto Kit
- Peroxidase Stain
- Ziehl Nelson
- Methylene Blue
Fronine Parasitology Stain kit.
Fronine lab supplies has released a new , and very competitively priced, Proto Stain Kit.
Proto stain is a permanent faecal smear stain comprising a modified ZN stain and iron haematoxylin. Using this combination, Coccidia protozoa such as Isospora, Cryptosporidia and cyclospora appear pink after being taken up in the carbol fuchsin stain.
Other faecal protozoa are stained by the iron haematoxylin producing typically blue/black nuclei with contrasting blue/grey cytoplasma. These staining characteristics combined with size and other morphological characteristics are utilized to allow easier detection and identification of faecal protozoa.
This stain is not designed to for the detection of eggs or Helminth larvae identification, although it may assist in their detection.
Reagents
- Acetone
- Acid Alcohol
- Immersion Oil
Consumables
- Eppendorf Combitips and Safe Lock Tubes
- Autoclave Bags - Available in Small, Medium and Large
- Counting Chambers - Neubauer
- Inoculating Loops - Disposable, Sterile, Nichrome
- Pasteur Pipettes - 5 ¾”, 5 ¾” Cotton Plugged, 9”, 9” Cotton Plugged
- Transfer Pipettes - plastic, sterile and non sterile
- Urinalysis Slides If your buying the Kova slide , try ours. We have available a high quality 10 Chamber; square with grids slide at the most competitive price in Australia. Already the national contract urine slide of choice with Australia’s largest private pathology providers.
- Uristrips - (for quantitive bacterial cell counts in urine)
- Sterile Saline Tubes
- Disposable Latex Examination Gloves – Fronine imports and offers an excellent quality, TGA approved , disposable glove. Available in S/M/L/XL all for the same price. Available in Powder Free , Lightly Powdered and Nitrile. We currently sell these gloves to Uni’s , Hospitals and Private Practices. Because we import them we can offer this excellent quality glove range at an economical price. Bulk and contract pricing available upon request
- Faeces Jars - also available prefilled with FAA Fixative
Mountants
- Ultramount – # 1 through 9 : Refractive index 1.515. This is acrylate/methacrylate based with Xylene as the solvent. Fast drying [touch dry in 3-4mins] and permanent [coverslips are difficult to remove]. The numbers relate to viscosity. No 1 is very thick and No 9 thin. Please note the most common viscosity is No 4, which is equivalent to Eukitt. However Ultramount No 9 has now been released, which is equivalent to Medite’s Pertex, and a mountant especially formulated for automatic cover-slipping machines.
- Aquamount – This is a water based mountant and is used for special staining techniques where the use of solvents removes the component being stained. This is not a permanent mountant. Otherwise known as Apathy’s mountant.
- DPX – see NorMount below
- Safety Mount – No 4 and 7 : Is again Perspex based but with Histolene as the solvent. If one is clearing with Histolene [our Xylene replacement solvent] then this mountant is better suited. Also available in our popular Safe-T-Mount squeeze tubes.
- Fastmount – Is also styrene based but fast drying. Touch dry in 10 mins. Excess mountant is easily removed from a coverslipped slide after a drying period, using a blade. Coverslips a fairly easy to remove after they have dried with mild heat and Xylene.
- Normount – Is styrene based and the equivalent of Depex or D.P.X., thick in consistency and slow drying. Touch dry in 20 mins minimum.
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