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Eppendorf twin.tec PCR Plates are manufactured
utilizing "twin-shot technology," an innovative
injection mold process. This unique method combines two of
the best performing materials: virgin polypropylene is used
for the wells; polycarbonate, an especially rigid material,
forms the plate deck and skirt.
Polypropylene provides the best inert, non-wetting
surface that prevents DNA, RNA, and enzymes from binding,
and it improves recovery from wells even when working with
minute volumes. Polycarbonate provides mechanical stability,
and it facilitates handling of the Eppendorf twin.tec PCR
plates. The enhanced stiffness offers improved plane parallelism
in the plate deck before, during, and after PCR, and it ensures
a more secure seal. Bending or curving of the skirt is virtually
eliminated, making twin.tec PCR Plates the ideal choice for
use with automation or robotics.
Eppendorf twin.tec PCR Plates also offer a
20% reduction in wall thickness compared to standard thin-walled
tubes. This provides a snug fit for the well against thermal
blocks, and it ensures virtually undisturbed heat transfer
from the block to the sample. Raised well rims optimize sealing
effectiveness and reduce the risk of cross-contamination.
All commonly used sealing methods—cap strips, adhesive
films, and foils—can be applied to Eppendorf twin.tec
PCR Plates for ultimate protection against evaporation and
heat sealing. |
| Applications
- PCR experiments
- Cycle sequencing
Product features
- One-piece design: polycarbonate and
polypropylene for optimum performance
- Improved plane parallelism
- Manufactured for rigidity
- Improved well-to-well tolerance
- Extremely thin-walled for optimum
heat transfer into the sample
- Fits most thermal cyclers
- Cut-away corner and alphanumeric grid
referencing
- Raised well rims for effective sealing
and reduced risk of cross-contamination
- Autoclavable
- Certified to be free of any detectable
human DNA, DNase, RNase, and PCR inhibitors*
Additional product features of
skirted plates
- Reduced bending or curving of skirt
- Compatible with automated systems
- Skirt can be labeled or bar-coded
- Can be used with the MegaBACE capillary
sequencer and most thermal cyclers
- Stackable
- Eight holes located in the skirt facilitate
plate positioning and removal from the thermal cycler block
- Low profile design for enhanced PCR
efficiency
- 150 µl well volume (when used
with cap strips)
- Within SBS footprint recommendations
(127.76 ±0.25 mm x 85.47 ±0.25 mm)
Additional product features of
semi-skirted plates
- "Semi" skirt can be labeled
or bar-coded
- Compatible with ABI GeneAmp 9700 and
most thermal cyclers
- 250 µl well volume (when used
with cap strips)
*Certificate, test procedures, and detailed
information available upon request.
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