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The Eppendorf Multiporator, in combination with the specially designed electroporation buffers, is the optimum balanced system for the efficient and gentle electroporation of eukaryotic cells.
The Multiporator uses extremely short pulses for the electroporation of eukaryotic cells. This Soft Pulse™ Technology produces exceptionally high survival rates.
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The transfection rates, which are particularly high for mammalian cells, are ensured by an optimally regulated system. The Multiporator is the only device on the market with a microprocessor-controlled discharge for very high reproducibility.
The special buffer system enables electro-deformation of the cells for membrane permeation at specific locations, thus highly sophisticated transformation experiments can be performed. Two optional modules expand the applicational range of the Multiporator to include the transformation of bacteria, yeasts and cell fusion. This compact device combines excellent operating comfort with the outstanding safety levels provided by the built-in cuvette chamber.
Product features
- Patented electronics* ensure accurate performance of the pulse parameters, independent of the sample resistance
- Soft Pulse Technology prevents any negative effect on cells (changes in pH values, the release of aluminum, electrophoresis of the cell content)
- An innovative, hypoosmolar buffer system boosts transfection efficiency for eukaryotes
- RS-232 interface
Technical Specifications
| Eukaryotic Module |
| Area of application: |
Electroporation of animal and human cell
lines, primary cells, plant cells, and oocytes |
| Pulse voltage: |
20–1,200 V |
| Time constant: |
15–500 µs, in 5 µs increments |
| Pulse form: |
Exponentially diminishing |
| Multiple pulsing: |
1–99, with 1 min. time interval |
| Cuvettes: |
Aluminum, sterile; gap width: 1 mm, 2
mm, 4 mm |
| Buffer: |
Hypo- and isoosmolar electroporation
buffer; sterile,
mycoplasm- and pyrogen-free |
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| Bacteria Module |
| Area of application: |
Electroporation of gram-positive and
gram-negative bacteria, and yeast |
| Pulse voltage: |
200–2,500 V |
| Pulse form: |
Exponentially diminishing |
| Time constant: |
5 ms (nominal) |
| Resistance: |
600 Ohm |
| Capacitor: |
10 µF |
| Special feature: |
Electronic safety switch for eliminating
short-circuits |
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| Fusion Module |
| Area of application: |
Electrofusion of mammalian cells, plant
cells, and oocytes |
| Pulse voltage: |
5–300 V |
| Pulse width: |
15–500 µs, in increments of
5 µs |
| Pulse form: |
Square-wave pulse |
| Multiple pulsing: |
1–99, in time intervals of 1 sec.
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| Sinus voltage: |
1–10 Vp,
symmetrically to 0 V |
| Frequency: |
2 MHz sinus |
| Time Range: |
0–30 sec. before and after pulses
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