Mastercycler ep: a system that goes farther

Mastercycler ep gradient, Mastercycler ep gradient S, and Mastercycler ep 384

Faster speeds, highest precision, more user-friendliness and absolute reliability united within a flexible concept: this is the definition of the Mastercycler ep system. In the past few years nucleic acid research has been developing dynamically. The same applies to the capabilities required of PCR Systems.

To keep pace with these technological advances, the challenges of tomorrow must be considered today. The Mastercycler ep system is today’s answer for the future.

With the Mastercycler ep system Eppendorf looks to support those working in a technologically advanced fashion with experience and a clear commitment to innovation.

Faster, more reliable, more flexible—and still able to adjust to future developments despite tighter budgets—that’s exactly how a thermal cycler should be today!

Product features of the Mastercycler ep

• Extremely fast heating and cooling rates
• Gradient blocks with SteadySlope™ technology
• Intuitive graphic programming
• Can be used as a stand-alone device, a “Mini-Satellite” system, or a PC-controlled network
• Heated lid with integrated ESP technology (Electronic Sample Protection)
• Optional motorized lid for integration into fully automated environments
• Licensed for PCR
• 2 year warranty

When heated lids learn to think

ESP heated lid

Eppendorf calls it ESP: Electronic Sample Protection. Users of the Mastercycler ep will regard it as the most modern heated lid technology. With conventional thermal cyclers, the heated lid immediately applies the necessary pressure required to press the PCR tubes into the block when it is closed. With sensitive applications, where it is important to suppress the condensation in the upper tube area from the start, the lid is heated up prior to beginning the actual PCR. ESP technology prevents undesired heating of the sample prior to PCR. The timing of the descent and the closing pressure of the ESP heated lid are controlled electronically.

Once the set temperature is reached, the lid automatically lowers onto the tube/plate with optimal pressure applied. As a result, the samples are optimally protected during the heating process.

The ESP heated lid performs an automatic tube height adjustment for all sample tubes and PCR plates. The optimal closing pressure is thus independently calculated and selected in such a way that evaporation is effectively minimized.

In other words, close the lid and let Mastercycler ep take care of everything else!

Product features of the ESP heated lid

• Space-saving sliding lid
• No preheating of the sample
• Minimized formation of non-specific primer dimers
• Complete prevention of condensation
• Automatic tube height adjustment
• Simple, one-handed, energy-saving operation
• Available with
• Manual lid
• Motorized lid for automated use in HTS labs

Three block formats

Mastercycler ep gradient Freely programmable temperature gradient over 12 rows A gradient range of up to 20°C Robust, specially coated aluminum block Mastercycler ep gradient S Highest temperature control speed due to silver block: up to 6°C/s Freely programmable temperature gradient over 12 rows A gradient range of up to 24°C Mastercycler ep 384 Freely programmable temperature gradient over 24 rows A gradient range of up to 20°C Optimized tube fit for the fastest heat transition: as with the 96-well blocks, the plates rest tightly in the block and come in direct contact with the walls

SteadySlope Technology

• Identical heating and cooling rates for all wells—even in gradient operation
• Assures reliable protocol transfer from gradient to normal operation

Certified quality

• Individual and informative quality control certificates
• Calibration according to national and international standards: UKAS/NPL (United Kingdom), DKD/PTB (Germany), NIST (USA)

Three cofigurations

Operation by control panel or PC software

Control Panel 1/4 VGA color display Microsoft® Windows® Explorer-like user administration More than 100 user-defined folders 16 MB memory capacity for more than 700 programs External memory option via multimedia cards Control of up to 5 different thermal modules in the “Mini-Satellite” system: saves time and money

Mastercycler ep CycleManager PC software for control of one or more thermal modules Network of up to 30 thermal modules Modern satellite concept via CAN-Bus/USB interfaces PC-controlled network

Stand-alone system

Equipped for the future from head to toe

One software—many possibilities

Product features for program and user administration

• One administrator to manage users and service functions
• Report file for each run, incl. total temperature profiling
• Easy addition of new users
• Password protection against unauthorized login
• Ability for Mastercycler ep users to change their password, no administrator access to user passwords
• Development, testing and validation of the software using certified standards

Product features for the generation of programs

• lHighest degree of flexibility, intuitive graphic programming
• Variable ramp rates
• Emulation mode for temperature control speed and protocols of the Mastercycler family
• Time and temperature increments
• Pause command
• Programmable alarm sound
• Freely configurable auto-restart dialogue